Related Experiment Video
Updated: Jul 4, 2026

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
Bacillus stearothermophilus for thermophilic production of L-lactic acid
H Danner1, M Neureiter, L Madzingaidzo
1Institute for Agrobiotechnology Tulln (IFA-Tulln), Department for Environmental Biotechnology, Konrad Lorenz Str. 20, A-3430 Tulln, Austria. danner@ifa1.boku.ac.at
Abstract:
A process for the continuous production of high purity L-lactic acid in a membrane bioreactor at 65 degrees C has been developed. Two different Bacillus stearothermophilus strains have been tested in batch experiments. Lactic acid yields are between 60 and more than 95% of theoretical yields. The amounts of ethanol, acetate, and formate formed varied between 0 and 0.4, 0 and 0.1, and 0 and 0.5, respectively (mol/mol glucose). All byproducts are valuable and may be separated easily by rectification of the fermentation broth. Complete cell retention enables high volumetric productivity (5 g/Lh), and a minimum of growth supplements. The high temperature of 65 degrees C allows the autoselective fermentation without problems with contamination.
Related Concept Videos
Production of Organic Acids
Microbes in Food Production
Hyperthermophilic Bacteria
Microbes in the Production of Fermented Foods
Production of Antibiotics
Scale-Up Processes

