Related Experiment Video
Updated: Jul 4, 2026
Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
Published on: September 17, 2012
Investigation of PrPC metabolism and function in live cells : methods for studying individual cells and cell
Cathryn L Haigh1, David R Brown
1Department of Pathology and Mental Health Research Institute of Victoria, University of Melbourne, Melbourne, Australia.
Abstract:
Prion protein (PrP)(C) expression levels and protein localization are known to be affected by factors such as metal ions and oxidative stress. By the development of a green fluorescent protein (GFP)-PrP(C) fusion protein, the movement of PrP can be followed in real time. Furthermore, alterations in cellular metabolism can be detected while cells are still viable. The internalization response of PrP to 20 microM manganese (Mn) in divalent metal ion-depleted media is used to demonstrate the movement of GFP-tagged proteins in live cells and real time. A live cell microtiter plate assay shows that PrP null cells are less capable of dealing with Mn-induced oxidative stress. In addition, this chapter outlines several complementary techniques for studying live cells and GFP fusion proteins.
