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Chronocoulometric techniques in the study of computerized enzyme electrode systems
1Center for Biotechnology, George S Wise Faculty of Life Science, Tel-Aviv University, Ramat Aviv 69978, Israel.
Biotechnology and Bioengineering
|February 1, 1987
Summary
This study explores two-substrate enzyme electrodes using chronocoulometry for enhanced sensitivity. The research successfully retrieved kinetic and transport parameters for glucose electrodes, demonstrating improved analytical capabilities.
Area of Science:
- Electrochemistry
- Enzyme Electrodes
- Biosensors
Background:
- Two-substrate enzyme electrodes offer potential for enhanced sensitivity.
- Chronocoulometry is a technique that can improve electrode performance.
- Understanding transport and kinetics is crucial for enzyme electrode optimization.
Purpose of the Study:
- To theoretically and experimentally investigate the electrochemical transient of two-substrate enzyme electrodes.
- To demonstrate the utility of chronocoulometric mode for retrieving kinetic and transport parameters.
- To evaluate a glucose electrode's performance with different cosubstrates.
Main Methods:
- Theoretical analysis using digital simulation of kinetics and transport equations.
- Experimental testing of a glucose oxidase-based electrode.
- Utilizing a computerized electrochemical system for control and data acquisition.
Main Results:
- Chronocoulometric operation enhances electrode sensitivity.
- Digital simulation accurately predicted the theoretical chronocoulometric response.
- The glucose electrode showed measurable responses with both air oxygen and p-benzoquinone.
Conclusions:
- Chronocoulometry is a valuable technique for studying two-substrate enzyme electrodes.
- The developed methods allow for the retrieval of key kinetic and transport parameters.
- Enzyme electrodes show promise for electrochemical sensing applications.
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