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Related Experiment Video

Updated: Jul 4, 2026

Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites
08:26

Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites

Published on: February 14, 2020

Transfected Eimeria tenella could complete its endogenous development in vitro.

T Y Shi1, X Y Liu, L L Hao

  • 1Parasitology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China.

The Journal of Parasitology
|June 26, 2008
PubMed
Summary

Eimeria tenella can express foreign genes during its development in cell culture. This finding supports the development of Eimeria spp. as a vector for oral vaccines.

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Area of Science:

  • Veterinary Parasitology
  • Molecular Biology
  • Vaccine Development

Background:

  • Live attenuated coccidiosis vaccines offer potential as carriers for expressing foreign antigens.
  • Eimeria tenella is a significant poultry pathogen.
  • Developing novel vaccine delivery systems is crucial for animal health.

Purpose of the Study:

  • To investigate the in vitro expression of an exogenous gene by Eimeria tenella.
  • To assess the feasibility of using Eimeria tenella as a gene expression vector.

Main Methods:

  • Transfection of Eimeria tenella sporozoites with the yellow fluorescent protein (YFP) gene.
  • Inoculation of transfected sporozoites into primary chicken kidney cells (PCKCs).
  • Observation of YFP expression throughout the parasite's developmental stages using fluorescence microscopy.

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Last Updated: Jul 4, 2026

Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites
08:26

Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites

Published on: February 14, 2020

Transient Transduction of the Strobilated Forms of Echinococcus granulosus
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Transient Transduction of the Strobilated Forms of Echinococcus granulosus

Published on: September 16, 2022

Main Results:

  • Fluorescent Eimeria tenella were observed as early as 15-20 hours post-inoculation.
  • YFP expression was detected throughout schizogony and gametogony stages.
  • Fluorescent oocysts were identified, with higher fluorescence intensity in the nucleus.

Conclusions:

  • Eimeria tenella can successfully express an exogenous gene throughout its endogenous development in vitro.
  • This demonstrates the potential of Eimeria spp. as a vector for delivering foreign antigens.
  • Facilitates further research into Eimeria spp. vector vaccines for oral administration.