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[The expression of fetuin-A and its pathological significance in fulminant hepatic failure in mice]
Pan Zhang1, Xue-gong Fan, Wang Haichao
1Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, China.
Objective:
To explore the dynamic changes of fetuin-A expression and the influences of the changes on liver damage, hepatocyte apoptosis and inflammation in a mouse fulminant hepatic failure (FHF) model.
Methods:
The changes of fetuin-A expression were investigated by semi-quantitative RT-PCR and Western blot. Immunohistochemical staining was used in TNFa and fetuin-A detection. Hepatocyte apoptosis was detected by TUNEL.
Results:
Fetuin-A mRNA expression decreased after the FHF model was established for 3 hours (compared with the normal group, P less than 0.01), while the protein expression decreased after nine hours (compared with the normal group, P less than 0.01). Fetuin-A expressions were negatively correlated with the liver pathological scores and TNFa levels.
Conclusion:
In our mouse FHF model, fetuin-A is a possible protective factor for liver damage.
