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Related Experiment Video

Updated: Jul 4, 2026

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

Human papillomavirus type 16 E7 oncoprotein associates with E2F6.

Margaret E McLaughlin-Drubin¹, Kyung-Won Huh, Karl Münger

¹Department of Medicine, Infectious Diseases Division, The Channing Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.

Journal of Virology

|June 27, 2008

Summary

Human papillomavirus (HPV) E7 protein disrupts E2F6

Area of Science:

Molecular biology
Virology
Cell cycle regulation

Background:

Papillomavirus replication is linked to epithelial cell differentiation.
Human papillomavirus (HPV) E7 protein drives aberrant cell cycle progression.
E2F6 acts as a repressor, slowing S-phase entry.

Purpose of the Study:

Investigate HPV E7's interaction with E2F6.
Determine if viral oncoproteins can inactivate E2F6's repressive function.
Understand the role of E2F6 inactivation in HPV-infected cells.

Main Methods:

Co-immunoprecipitation assays to detect protein interactions.
Western blotting to assess protein levels and activity.
Immunofluorescence staining to visualize protein localization and complex formation.

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RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Published on: March 11, 2014

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Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

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Published on: March 8, 2012

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

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Published on: July 23, 2010

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Published on: March 11, 2014

Main Results:

HPV E7 proteins associate with and inactivate E2F6's transcriptional repression.
Simian virus 40 T antigen and adenovirus E1A also interact with and inactivate E2F6.
E7-expressing cells exhibit reduced E2F6/polycomb complex staining, partly due to E7-E2F6 association.

Conclusions:

Viral oncoproteins, including HPV E7, subvert E2F6-mediated cellular defense mechanisms.
Inactivation of E2F6 by HPV E7 may lead to prolonged S-phase competence in infected cells.
E2F6's role in polycomb complexes and cell fate maintenance is disrupted by viral infection.