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Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Chromatin Structure Regulates pre-mRNA Processing

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Related Experiment Video

Updated: Jul 4, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Conserved RNA secondary structures promote alternative splicing.

Peter J Shepard¹, Klemens J Hertel

¹Department of Microbiology and Molecular Genetics, Institute for Genomics and Bioinformatics, University of California at Irvine, Irvine, California 92697-4025, USA.

RNA (New York, N.Y.)

|June 27, 2008

Summary

RNA secondary structures influence alternative splicing by masking splice sites. This study identifies conserved RNA structures in the human genome associated with alternative splice-site selection, highlighting their role in gene regulation and disease.

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Last Updated: Jul 4, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Published on: April 22, 2021

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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Area of Science:

Molecular Biology
Genomics
Bioinformatics

Background:

Pre-messenger RNA (pre-mRNA) splicing is a critical process for gene expression, carried out by the spliceosome.
Alternative splicing in higher eukaryotes generates diverse protein isoforms from a single gene transcript.
Splice-site selection depends on various factors, including splice-site strength, regulatory proteins, and pre-mRNA synthesis.

Purpose of the Study:

To investigate the role of RNA secondary structures in alternative splicing.
To identify conserved RNA structural elements in the human genome associated with alternative splice-site selection.

Main Methods:

Analysis of structural and functional conservation of RNA elements.
Identification of RNA structures linked to alternative splicing events in the human genome.

Main Results:

Discovery of specific RNA structure elements within the human genome that are associated with alternative splice-site selection.
Demonstration of the frequent involvement of these RNA structures in alternative splicing.

Conclusions:

RNA secondary structure formation is a significant regulatory mechanism influencing alternative splicing.
These RNA structures play a role in regulating gene expression and are implicated in disease pathogenesis.