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Updated: Jul 4, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
Published on: June 30, 2018
Focus-of-attention from local color symmetries
1Faculty of Technology, Neuroinformatics Group, University of Bielefeld, PO Box 100131, Germany. gheidema@techfak.uni-bielefeld.de
Abstract:
In this paper, a continuous valued measure for local color symmetry is introduced. The new algorithm is an extension of the successful gray value-based symmetry map proposed by Reisfeld et al. The use of color facilitates the detection of focus points (FPs) on objects that are difficult to detect using gray-value contrast only. The detection of FPs is aimed at guiding the attention of an object recognition system; therefore, FPs have to fulfill three major requirements: stability, distinctiveness, and usability. The proposed algorithm is evaluated for these criteria and compared with the gray value-based symmetry measure and two other methods from the literature. Stability is tested against noise, object rotation, and variations of lighting. As a measure for the distinctiveness of FPs, the principal components of FP-centered windows are compared with those of windows at randomly chosen points on a large database of natural images. Finally, usability is evaluated in the context of an object recognition task.
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