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The function of a colloid in liver cold-storage preservation
A Ar'Rajab1, B Ahrén, R Sundberg
1Department of Surgery, Lund University, Sweden.
Transplantation
|July 1, 1991
Summary
Colloids do not prevent hypothermia-induced cell swelling during liver cold storage but do prevent interstitial edema. This edema prevention improves overall liver function and bile production after preservation.
Area of Science:
- Organ transplantation
- Surgical preservation
Background:
- The role of colloid in liver preservation during cold storage requires further clarification.
- Understanding colloid's impact is crucial for optimizing organ viability.
Purpose of the Study:
- To investigate the effects of colloid on cell swelling, liver weight, and bile production in cold-stored rat liver tissue slices and isolated rabbit livers.
- To determine if colloids mitigate negative effects of cold storage preservation.
Main Methods:
- Rat liver tissue slices and isolated rabbit livers were cold-stored for 24 hours.
- Preservation solutions included UW solution and Perfadex, with and without colloids (hydroxyethyl starch, dextran).
- Evaluated total tissue water (TTW), liver weight changes, and bile production.
Main Results:
- Colloids did not prevent hypothermia-induced cell swelling in rat liver slices preserved in Perfadex.
- In rabbit livers, colloids significantly reduced weight gain (interstitial edema) in Perfadex preservation.
- Colloids partially restored bile production in Perfadex-preserved rabbit livers.
Conclusions:
- Colloids do not prevent hypothermia-induced cell swelling but effectively prevent interstitial edema in cold-stored livers.
- Colloid addition improves liver function, particularly bile production, after cold storage preservation.
- These findings highlight the importance of colloid in optimizing organ preservation solutions.