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Updated: Jan 9, 2026
Neurogenesis Using P19 Embryonal Carcinoma Cells
Published on: April 27, 2019
Mitosis-dependent protein expression in neuroblastoma cell line N1E-115
Amedeo A Azizi1, Sung-Ung Kang, Angelika Freilinger
1Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Wahringer Gurtel 18-20, Vienna, Austria.
Abstract:
Systematic work on differential protein expression in mitosis is limited, and we therefore used neuroblastoma cells (N1E-115) incubated with either colcemid or nocodazole to arrest mitosis. Proteins were identified by MALDI-TOF/TOF and nano-LC-ESI-MS/MS with subsequent quantification of spot volumes with specific software. Immunoblotting was used for verification of selected proteins. Levels of 10 individual proteins were increased and levels of 6 proteins were decreased concordantly by both treatments. These proteins were constituents of heat shock and chaperone, cytoskeleton, proteasomal, heterochromatin, and DNA replication signaling as well as housekeeping and metabolic systems. Identification of mitosis-dependent proteins is of importance for the interpretation of previous work and for designing future experiments.
Insights
This study identified proteins with altered expression during mitosis in neuroblastoma cells using colcemid and nocodazole. These proteins are involved in crucial cellular processes, aiding future research on cell division.
Area of Science:
- Cell Biology
- Molecular Biology
- Proteomics
Background:
- Differential protein expression during mitosis remains underexplored.
- Understanding mitosis-specific protein changes is crucial for interpreting cell cycle research.
Purpose of the Study:
- To systematically identify proteins with altered expression levels during mitosis.
- To characterize the functional roles of mitosis-dependent proteins in neuroblastoma cells.
Main Methods:
- Mitotic arrest of N1E-115 neuroblastoma cells using colcemid or nocodazole.
- Protein identification via MALDI-TOF/TOF and nano-LC-ESI-MS/MS.
- Quantification using specialized software and verification by immunoblotting.
Main Results:
- Identified 10 proteins with increased and 6 proteins with decreased levels concordantly under both mitotic arrest conditions.
- These proteins are involved in heat shock/chaperone, cytoskeleton, proteasomal, heterochromatin, DNA replication, and metabolic pathways.
- Confirmed changes for selected proteins using immunoblotting.
Conclusions:
- Provides a foundational dataset of mitosis-regulated proteins in neuroblastoma cells.
- Highlights the importance of these proteins in cell cycle progression and cellular functions.
- Facilitates more accurate interpretation of existing studies and guides future experimental design in cell division research.
