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Updated: Jul 4, 2026

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
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Multifunctional surfaces with discrete functionalized regions for biological applications.

Moniraj Ghosh1, Christina Alves, Ziqiu Tong

  • 1Department of Chemical & Biomolecular Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|June 28, 2008
PubMed
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This study presents a new method for creating multifunctional surfaces with distinct protein patterns on a PEG-functionalized background, enabling precise control over cell interactions and biological studies.

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Cell Biology

Background:

  • Controlling surface properties is crucial for biological applications.
  • Existing methods lack precise control over protein patterning and functionality.
  • Understanding cell behavior on patterned surfaces requires advanced fabrication techniques.

Purpose of the Study:

  • To develop a method for creating multifunctional surfaces with discrete protein patches.
  • To enable precise control over protein presentation for biological studies.
  • To demonstrate the utility of these surfaces in studying cellular interactions.

Main Methods:

  • Microcontact printing with octadecyltrichlorosilane to define active regions.
  • Back-filling with PEG-silane to create passive regions.

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Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution
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Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution

Published on: October 31, 2014

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Last Updated: Jul 4, 2026

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
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Published on: October 25, 2018

A Method of Targeted Cell Isolation via Glass Surface Functionalization
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Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution
08:23

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Published on: October 31, 2014

  • Utilizing microfluidics for protein delivery and patterning.

    • Main Results:

    • Successfully created trifunctional surfaces with distinct protein and PEG regions.
    • Demonstrated reproducible protein surface densities within patterned patches.
    • Showcased cellular interactions including cell capture, sorting, and adhesion under shear stress.

    Conclusions:

    • The developed method allows for the creation of versatile, protein-patterned surfaces.
    • These surfaces are valuable tools for fundamental research in cell adhesion and differentiation.
    • Potential applications include advanced biosensors and controlled cell culture systems.