Related Experiment Video
Updated: Jul 4, 2026

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
Published on: January 31, 2025
Generation and characterization of a single-chain Fv antibody against G, a hedgehog signaling pathway transcription
Silja Laht1, Kati Meerits, Harri Altroff
1Celecure AS, Akadeemia tee, Tallinn, Estonia.
Abstract:
Gli3 is a key regulator of development, controlling multiple patterning steps. Here we report the generation of a scFv antibody specific to the repressor domain of human Gli3. We show that this scFv retains the binding capacity of its parent anti-Gli3 monoclonal antibody derived from hybridoma clone 5E1. When expressed in mammalian cells, the anti-Gli3 scFv co-localizes with intracellular Gli3. Immunocytochemical staining of the intrabody in Gli3-positive TM4 cells shows a distinct perinuclear cytoplasmic localization. Such a scFv constitutes a useful tool for studying transcriptional regulation of the hedgehog pathway in mammals and offers a starting point for developing novel Gli-related therapeutic intrabodies.
Related Concept Videos
Hedgehog Signaling Pathway
Hedgehog Signaling Pathway
TGF - β Signaling Pathway

