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[Screening peptides binding specifically to large intestinal cancer LoVo cells from phage random peptide library]
Kang-Xiong Liao1, Xue-Qing Yao, Cheng-Tang Wu
1Department of General Surgery, Nanfang Hosptial, Southern Medical University, Guangzhou 510515, China.
Objective:
To screen the polypeptides specifically binding to human large intestinal cancer LoVo cells from a phage-displayed peptide library for potential use as targeting vectors for large intestinal cancer therapy.
Methods:
With the LoVo cells as the target cells and human normal large intestinal mucosal epithelial cells as the absorber cells for subtraction biopanning from a c7c phage-display peptide library, the positive phage clones were identified by enzyme-linked immunosorbent assay (ELISA) and immunofluorescence detection. The amino acid sequences of the identified peptides were deduced by DNA sequencing.
Results:
After 3 rounds of screening, 5 positive phage clones showing specific binding to LoVo cells and containing conserved motif RPMP were obtained from the 20 randomly selected clones.
Conclusion:
Specific peptide against large intestinal cancer cells can be obtained from a phage-display peptide library for use as potential vectors for targeting therapy of large intestinal cancer.
