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Updated: Jul 4, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
[High-performance liquid chromatography-mass spectrometry for determining olmesartan in human plasma]
Shi-Heng Chen1, Cui-Fang Wu, Ben-Mei Chen
1Department of Pharmacy, Liling Hospital of Traditional Chinese Medicine, Liling 420000, China.
Objective:
To establish a simple and rapid HPLC-MS method for determining the contents of olmesartan in human plasma.
Methods:
Plasma were precipitated with trifluoroacetic acid, then analyzed on an HyPurity C(18) column (150 mm 2.1 mm, 5 microm). Samples at 40 degrees celsius;. The mobile phase consisted of water-methanol- acetonitrile(14:60:26) with a flow rate of 0.22 ml/min.
Results:
The lower limit of qualification was 25 microg/L. The calibration curve was linear over the range of 25-3200 microg/L (r=0.9998), with the intra-day and inter-day RSD less than 15%.
Conclusion:
The method is sensitive, rapid and suitable for the study of pharmacokinetics and bioavailability of olmesartan.
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