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Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

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Weighted filtered backprojection for quantitative fluorescence optical projection tomography.

A Darrell¹, H Meyer, K Marias

¹BMI Laboratory, Institute of Computer Science, Foundation for Research and Technology-Hellas, Vassilika Vouton, PO Box 1385, GR 711 10 Heraklion, Greece.

Physics in Medicine and Biology

|June 28, 2008

Summary

Reconstructing fluorescence optical projection tomography (OPT) images requires accounting for physical factors like distance and defocus. A new weighted filtered backprojection (WFBP) algorithm improves quantitative accuracy by incorporating these effects.

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Area of Science:

Biomedical Imaging
Optical Physics
Image Reconstruction

Background:

Fluorescence optical projection tomography (OPT) image reconstruction is a developing field.
Standard filtered backprojection (FBP) algorithms do not account for physical factors influencing fluorescence OPT image intensity.
These factors include fluorophore distance from the objective and image defocus, impacting quantitative accuracy.

Purpose of the Study:

To develop a more accurate image reconstruction algorithm for fluorescence OPT.
To address the limitations of traditional FBP by incorporating physical models of image formation.

Main Methods:

A comprehensive model of fluorescence image formation was developed, considering isotropic emission and defocus.
This model yielded a weighting function to modify the filtered backprojection (FBP) algorithm.
The modified algorithm, termed weighted filtered backprojection (WFBP), was implemented and tested.

Main Results:

The WFBP algorithm demonstrated improved quantitative accuracy compared to standard FBP.
Testing with simulated data showed the effectiveness of the weighting function.
Experimental data from a fluorescent microsphere phantom validated the algorithm's performance.

Conclusions:

The developed weighted filtered backprojection (WFBP) algorithm provides more quantitative reconstructions in fluorescence OPT.
Accounting for physical effects like isotropic emission and defocus is crucial for accurate fluorescence tomography.
This advancement has implications for precise 3D imaging in biological research.