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Lineage Commitment

Lineage Commitment

Commitment is the process whereby stem cells:

Methods of Nuclear Reprogramming

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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.

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Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...

Introduction to Nuclear Reprogramming

Introduction to Nuclear Reprogramming

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Related Experiment Video

Updated: Jul 4, 2026

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons

Published on: May 12, 2014

Lineage-specific reprogramming as a strategy for cell therapy.

Radbod Darabi¹, Rita C R Perlingeiro

¹Department of Developmental Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.

Cell Cycle (Georgetown, Tex.)

|June 28, 2008

Summary

This summary is machine-generated.

Embryonic stem cells offer therapeutic potential but face challenges like teratoma formation. This study successfully generated skeletal muscle progenitors from mouse embryonic stem cells by overexpressing a master regulator, overcoming lineage restriction.

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Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

Published on: July 29, 2015

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Last Updated: Jul 4, 2026

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons

Published on: May 12, 2014

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

Published on: July 29, 2015

Area of Science:

Stem cell biology
Regenerative medicine
Developmental biology

Background:

Embryonic stem (ES) cells possess self-renewal and differentiation capabilities, making them promising for cell therapy.
Current therapeutic applications of human ES cells and induced pluripotent stem (iPS) cells are limited by teratoma formation and difficulties in isolating specific cell populations.

Purpose of the Study:

To address the challenges in deriving specific cell populations from ES cells for therapeutic use.
To develop a method for generating skeletal muscle progenitors from mouse ES cells.

Main Methods:

Focus on the derivation of skeletal muscle progenitors from mouse ES cells.
Employing a strategy of reprogramming lineage choices through the overexpression of a master regulator gene.

Main Results:

Successfully generated skeletal myogenic lineage from mouse ES cells.
Demonstrated the effectiveness of master regulator overexpression in directing ES cell differentiation towards a specific lineage.

Conclusions:

Overexpression of a master regulator is a viable strategy for reprogramming lineage choices in ES cells.
This approach facilitates the generation of specific therapeutic cell populations, such as skeletal muscle progenitors, from ES cells, potentially overcoming current limitations in cell therapy.