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Updated: Jul 4, 2026

High Throughput Single-cell and Multiple-cell Micro-encapsulation
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High Throughput Single-cell and Multiple-cell Micro-encapsulation

Published on: June 15, 2012

Drop-based microfluidic devices for encapsulation of single cells.

Sarah Köster1, Francesco E Angilè, Honey Duan

  • 1Department of Physics and School of Engineering and Applied Sciences, Harvard University, Cambridge, USA.

Lab on a Chip
|June 28, 2008
PubMed
Summary
This summary is machine-generated.

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This study introduces microfluidic devices for high-throughput single-cell analysis. These devices enable rapid detection of secreted molecules, like antibodies from hybridoma cells, while maintaining cell viability.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Traditional cell-based assays often require large volumes and can be time-consuming.
  • High-throughput analysis of individual cells in their microenvironment is challenging.
  • Developing adaptable microfluidic systems for diverse cell assays is an ongoing need.

Purpose of the Study:

  • To develop a modular microfluidic system for encapsulating, incubating, and manipulating single cells.
  • To demonstrate the rapid detection of secreted molecules from individual cells using picoliter droplets.
  • To showcase the potential for advanced cell sorting and analysis based on cellular secretions.

Main Methods:

  • Utilizing microfluidic devices to create picoliter aqueous drops containing individual cells within a carrier fluid.

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Last Updated: Jul 4, 2026

High Throughput Single-cell and Multiple-cell Micro-encapsulation
16:19

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Published on: June 15, 2012

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms
06:50

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

Published on: February 11, 2019

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Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells

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  • Employing a modular system with distinct devices for encapsulation, incubation, and manipulation.
  • Operating the system at high throughput rates (up to several hundred Hz).

    • Main Results:

    • Demonstrated successful encapsulation, incubation, and manipulation of single cells in picoliter drops.
    • Showed that single hybridoma cells in 33 pL drops secrete detectable antibody concentrations within 6 hours.
    • Confirmed that cells remain fully viable throughout the process.

    Conclusions:

    • The modular microfluidic system offers a robust, flexible, and adaptable platform for cell-based assays.
    • Picoliter droplet volumes facilitate rapid attainment of detectable molecular concentrations, enabling faster analysis.
    • These microfluidic devices pave the way for next-generation cell cytometers and sorters with enhanced functionality for single-cell analysis.