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Related Experiment Videos

An integrated optofluidic platform for Raman-activated cell sorting.

Adrian Y Lau1, Luke P Lee, James W Chan

  • 1Applied Physics and Biophysics Division, Lawrence Livermore National Laboratory, P.O. Box 808, L-211, Livermore, CA 94550, USA.

Lab on a Chip
|June 28, 2008
PubMed
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This study presents an integrated optofluidic Raman-activated cell sorting platform for label-free cell identification and sorting. The system enables automated, continuous sorting of individual cells using intrinsic Raman markers.

Area of Science:

  • Optofluidics
  • Spectroscopy
  • Cell Biology

Background:

  • Traditional cell sorting methods often require cell labeling, which can affect cell viability and function.
  • Label-free cell analysis offers a more direct and less invasive approach to cell characterization.
  • Advancements in microfluidics and laser spectroscopy enable sophisticated single-cell analysis.

Purpose of the Study:

  • To develop and demonstrate an integrated optofluidic platform for label-free cell sorting.
  • To combine microfluidic cell handling with laser tweezers Raman spectroscopy (LTRS) for cell identification and sorting.
  • To evaluate different optofluidic designs for optimal sorting efficiency.

Main Methods:

  • Integration of multichannel microfluidic devices with laser tweezers Raman spectroscopy (LTRS).

Related Experiment Videos

  • Utilizing hydrodynamic focusing and pinch-flow fractionation for cell manipulation.
  • Assessing laser trapping efficiency at various laser power levels and flow velocities.
  • Proof-of-principle demonstration using two leukemia cell lines.

    • Main Results:

    • Successful label-free identification and sorting of individual cells based on intrinsic Raman spectra.
    • Evaluation of two optofluidic designs, demonstrating the impact of design and flow velocity on trapping efficiency.
    • Demonstration of automated, continuous cell sorting capabilities.
    • Proof-of-concept achieved for sorting distinct leukemia cell lines.

    Conclusions:

    • The integrated optofluidic Raman-activated cell sorting (RACS) platform enables label-free cell identification and sorting.
    • This technology provides a foundation for automated cell sorting systems utilizing intrinsic Raman markers.
    • The developed platform holds potential for high-throughput analysis of individual cells in solution without labeling.