Related Experiment Video
Updated: Jul 4, 2026

A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation
Published on: June 3, 2020
A fermentor for study of sauerkraut fermentation
H P Fleming1, R F McFeeters, E G Humphries
1Food Fermentation Laboratory, U.S. Department of Agriculture, Agricultural Research Service, and North Carolina Agricultural Research Service, Department of Food Science, North Carolina State University, Raleigh, North Carolina 27695-7624.
This study details a novel fermentor for sauerkraut, revealing two distinct fermentation stages: a gaseous phase with rapid CO2 and acid production, followed by a non-gaseous phase. Nitrogen purging offers benefits like brine circulation and nutrient retention.
Area of Science:
- Food Science and Technology
- Microbiology
- Chemical Engineering
Background:
- Sauerkraut fermentation involves complex physical, microbiological, and chemical changes.
- Understanding these changes is crucial for optimizing sauerkraut production and quality.
Purpose of the Study:
- To design and construct a specialized fermentor for studying sauerkraut fermentation.
- To investigate the distinct stages and microbial dynamics during sauerkraut fermentation.
- To evaluate the impact of nitrogen purging on the fermentation process.
Main Methods:
- A custom fermentor was built with features for volume restriction, visual liquid level monitoring, and nitrogen purging.
- Fermentation processes were monitored for physical, microbiological, and chemical changes.
- The effects of nitrogen purging on brine circulation, CO2 removal, and anaerobiosis were assessed.
Main Results:
- Sauerkraut fermentation exhibited two distinct stages: a gaseous stage (hetero-fermentative bacteria, CO2 production, fructose to mannitol conversion) and a non-gaseous stage (homo-fermentative bacteria, increased lactic acid).
- Gas entrapment caused liquid level rises during the gaseous stage.
- Nitrogen purging facilitated brine circulation, CO2 removal, and maintained anaerobiosis, preserving ascorbic acid and color.
Conclusions:
- The designed fermentor effectively facilitates the study of sauerkraut fermentation dynamics.
- The two-stage fermentation process is characterized by specific microbial activities and chemical transformations.
- Nitrogen purging is a valuable technique for enhancing sauerkraut quality and process control.
Related Concept Videos
Microbes in the Production of Fermented Foods
Fermentation
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Microbes in Food Production
Microbial Fermentation
Microbes in Beverage Production
Production of Alcohol

