Related Experiment Video
Updated: Jul 4, 2026

Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
Published on: June 13, 2014
A microfibril-generating factor from the cellulase of Trichoderma reesei
L H Krull1, F R Dintzis, H L Griffin
1Northern Regional Research Center, Agricultural Research Service, U.S. Department of AgricultureThe mention of firm names or trade products does not imly that they are endorsed or recommended by the U.S. Department of Agriculture over other firms or similar products not mentioned, Peoria, Illinois 61604.
Abstract:
A combination of ionic strength reduction and diafiltration of Trichoderma reesei cellulate complex through a hollow fiber apparatus of 5000 molecular weight (MW) cutoff and subsequent passage of filtrate over a Spherogel-TSK 3000-SW column provided extracts that had the ability to generate microfibrils in filter paper and to disrupt filter paper and corn leaf tissue. Milligram quantities of material obtained from these extracts released small amounts of soluble carbohydrate from filter paper, required ferric iron for increased activity, and contained amino acids. Short fiber formation and disruption of filter paper during interaction with these extracts was enhanced by prior acid treatment and eliminated by prior base treatment. The amount of soluble carbohydrate hydrolyzed in 24 h from filter paper by whole cellulase complex was not changed by first disrupting the substrate with the extracts.
Related Concept Videos
Role of Microtubules in Cell Wall Deposition
Cell Motility through Blebbing
Blebbing Through the Matrix
In multicellular...
Cellulose and Pectic Polysaccharides
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...
Mechanism of Filopodia Formation
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
The Contractile Ring
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...
Cell-matrix's Response to Mechanical Forces
Anchoring junctions mechanically attach a cell to the...

