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Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
Published on: June 13, 2014
A microfibril-generating factor from the cellulase of Trichoderma reesei
L H Krull1, F R Dintzis, H L Griffin
1Northern Regional Research Center, Agricultural Research Service, U.S. Department of AgricultureThe mention of firm names or trade products does not imly that they are endorsed or recommended by the U.S. Department of Agriculture over other firms or similar products not mentioned, Peoria, Illinois 61604.
Biotechnology and Bioengineering
|March 1, 1988
Summary
Researchers purified Trichoderma reesei cellulase complex extracts. These extracts can disrupt plant tissues and generate microfibrils, with activity influenced by pH and ferric iron.
Area of Science:
- Biochemistry
- Enzymology
- Plant Science
Background:
- Trichoderma reesei is a key source of cellulase enzymes crucial for biomass degradation.
- Understanding the specific components and activities of cellulase complexes is vital for biotechnological applications.
Purpose of the Study:
- To isolate and characterize active components from the Trichoderma reesei cellulase complex.
- To investigate the ability of these components to interact with and modify plant cellulosic materials.
Main Methods:
- Purification of the cellulase complex using hollow fiber apparatus (5000 MWCO) and Spherogel-TSK 3000-SW chromatography.
- Assay of extract activity on filter paper and corn leaf tissue.
- Analysis of factors influencing activity, including pH, ferric iron, and soluble carbohydrate release.
Main Results:
- Purified extracts generated microfibrils and disrupted filter paper and corn leaf tissue.
- Activity was enhanced by ferric iron and prior acid treatment, but eliminated by base treatment.
- Small amounts of soluble carbohydrate were released, but the overall hydrolysis rate by the whole cellulase complex remained unchanged.
Conclusions:
- Specific components within the Trichoderma reesei cellulase complex possess tissue-disrupting and microfibril-generating capabilities.
- The activity of these components is modulated by environmental factors like pH and metal ions.
- These findings contribute to understanding enzyme-substrate interactions in cellulose breakdown.
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