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Updated: Jul 4, 2026
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Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
Use of surface-immobilized Trichoderma in batch and fed-batch fermentations
1Division of Biological Sciences, National Research Council of Canada, Ottawa, Canada, K1A 0R6.
Biotechnology and Bioengineering
|March 1, 1988
Summary
Immobilizing Trichoderma reesei on needle-punch polyester provides a stable, resident inoculum. This method reduces biomass in liquid cultures while maintaining significant enzyme activity for industrial applications.
Area of Science:
- Biotechnology
- Industrial Microbiology
- Enzyme Technology
Background:
- Trichoderma reesei Rut C30 is a key microorganism for industrial enzyme production.
- Immobilization techniques are crucial for enhancing microbial stability and reusability in bioprocesses.
- Reducing biomass in fermentation broths is desirable for downstream processing efficiency.
Purpose of the Study:
- To evaluate needle-punch polyester as a support for immobilizing Trichoderma reesei Rut C30.
- To compare the performance of immobilized Trichoderma in batch and fed-batch cultures against free mycelium.
- To assess the impact of immobilization on biomass generation and enzyme activity.
Main Methods:
- Immobilization of Trichoderma reesei Rut C30 on needle-punch polyester.
- Cultivation in batch and fed-batch fermentation systems.
- Comparison of biomass concentration and enzyme activity between immobilized and free cell cultures.
Main Results:
- Needle-punch polyester effectively immobilized Trichoderma reesei Rut C30.
- Immobilized cultures exhibited greater stability and reduced biomass generation in the liquid phase.
- Fed-batch fermentation with immobilized cells achieved approximately 80% of the activity of free cells, with enhanced stability and lower biomass concentration in the enzyme broth.
Conclusions:
- Needle-punch polyester is a suitable and effective support for Trichoderma reesei immobilization.
- Immobilization offers significant advantages in terms of culture stability and reduced biomass contamination.
- This approach holds promise for more efficient and stable industrial enzyme production.
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