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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...
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Continuous Renal Replacement Therapy (CRRT) is an essential intervention for patients experiencing severe kidney dysfunction. This therapy offers a continuous mechanism for removing fluids and toxins from the bloodstream, leveraging the patient’s blood pressure to facilitate filtration through a specialized filter. This method contrasts with intermittent dialysis, providing a gentler and more consistent removal of waste products and excess fluid, which is particularly beneficial in critically...

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Updated: Jul 4, 2026

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus
06:45

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Published on: June 2, 2023

A continuous affinity ultrafiltration process for trypsin purification.

J H Luong1, K B Male, A L Nguyen

  • 1Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada, H4P 2R2.

Biotechnology and Bioengineering
|April 20, 1988
PubMed
Summary
This summary is machine-generated.

A novel continuous process uses affinity chromatography and ultrafiltration to purify crude trypsin. This method achieves high purity and yield, offering a cost-effective solution for enzyme purification.

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Area of Science:

  • Biochemistry
  • Chemical Engineering
  • Biotechnology

Background:

  • Crude trypsin purification is essential for various applications.
  • Existing methods can be inefficient and costly.
  • Continuous processing offers potential advantages in enzyme purification.

Purpose of the Study:

  • To develop and test a continuous purification process for crude trypsin.
  • To utilize affinity chromatography and ultrafiltration for enzyme purification.
  • To improve the efficiency and reduce the cost of trypsin purification.

Main Methods:

  • Developed a continuous process combining affinity chromatography and ultrafiltration.
  • Used a water-soluble polymer with m-aminobenzamidine as a macroligand to bind trypsin.
  • Employed ultrafiltration membranes to retain the trypsin-macroligand complex.
  • Eluted bound trypsin using arginine or benzamidine.
  • Incorporated recirculation of eluant and macroligand.

Main Results:

  • Achieved a trypsin purification yield of 77%.
  • Reduced impurities to only 3%.
  • Demonstrated the feasibility of continuous enzyme purification using affinity ultrafiltration.
  • Showcased the reusability and long operative life of the affinity polymer.

Conclusions:

  • The developed affinity ultrafiltration process is effective for continuous trypsin purification.
  • This method significantly increases productivity and reduces purification costs.
  • The process offers a scalable and cost-efficient solution for enzyme purification.