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Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
Published on: February 7, 2022
Protein precipitate recovery using microporous membranes
A C Bentham1, M J Ireton, M Hoare
1Department of Chemical and Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, England.
Biotechnology and Bioengineering
|June 5, 1988
Summary
Microporous membranes can recover precipitated and soluble proteins. However, protein precipitation significantly reduces membrane flux and transmission, impacting recovery efficiency.
Area of Science:
- Biochemical Engineering
- Separation Science
- Membrane Technology
Background:
- Protein recovery is crucial in biotechnology and pharmaceutical industries.
- Microporous membranes offer potential for protein separation and purification.
- Understanding membrane performance with precipitated and soluble proteins is essential for process optimization.
Purpose of the Study:
- To investigate the performance of microporous membranes for recovering precipitated and soluble proteins.
- To analyze membrane flux rates and protein transmission under different experimental conditions.
- To elucidate the mechanisms behind flux decline and protein transmission in membrane systems.
Main Methods:
- Unstirred batch-cell studies were conducted to assess protein transmission.
- Cross-flow experiments were performed to evaluate membrane flux and transmission over time.
- Protein solutions and suspensions at varying pH levels were utilized.
Main Results:
- High soluble protein transmissions (80-100%) were achieved in unstirred batch-cell studies.
- A sharp decline in membrane flux was observed, particularly at the protein's isoelectric point (pH 4.6).
- The presence of precipitated protein further decreased flux rates, and cross-flow studies showed reduced protein transmission with flux decline.
Conclusions:
- Microporous membranes show promise for protein recovery, but flux decline is a significant challenge.
- Protein precipitation and interaction with the membrane surface critically affect membrane performance.
- Further research is needed to optimize membrane processes for efficient protein recovery, especially with precipitated proteins.
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