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Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii
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Foam separation of microbial cells.

S Parthasarathy1, T R Das, R Kumar

  • 1Department of Chemical Engineering, Indian Institute of Science, Bangalore-560 012, India.

Biotechnology and Bioengineering
|July 5, 1988
PubMed
Summary

This study introduces a novel foam separation method for Saccharomyces carlsbergensis cells without external agents. A predictive model accurately describes cell concentration changes during this innovative bioprocess.

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Area of Science:

  • Biochemical Engineering
  • Separation Science

Background:

  • Yeast cell separation from fermentation broth is crucial for bioprocessing.
  • Conventional methods often require external surfactants, increasing costs and complexity.

Purpose of the Study:

  • To develop and validate a model for batch foam separation of Saccharomyces carlsbergensis.
  • To predict cell concentration in foamate and bulk liquid over time.
  • To investigate the influence of operational parameters on separation efficiency.

Main Methods:

  • Batch foam separation technique applied to Saccharomyces carlsbergensis.
  • Development of a mathematical model incorporating interfacial and bulk liquid dynamics.
  • Assumption of linear equilibrium between interface and bulk concentrations.
  • Geometric modeling of foam bubbles as pentagonal dodecahedra to calculate interfacial area.

Main Results:

  • The developed model accurately predicts foamate cell concentration and bulk liquid depletion.
  • The model effectively explains the impact of bubble size and aeration rate on cell separation.
  • Demonstrated successful cell separation without the need for external surface-active agents.

Conclusions:

  • Batch foam separation is a viable, surfactant-free method for yeast cell recovery.
  • The predictive model provides valuable insights into foam separation mechanisms.
  • This approach offers a cost-effective and efficient alternative for bioprocess downstream processing.