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Updated: Jul 4, 2026

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Label-free DNA electrochemical sensor based on a PNA-functionalized conductive polymer.

S Reisberg¹, L A Dang, Q A Nguyen

¹Laboratoire Interfaces-Traitements-Organisation et Dynamique des Systèmes, Université Paris 7-Denis Diderot, associé au CNRS, UMR 7086, 1 rue Guy de la Brosse, Paris 75005, France.

|July 1, 2008

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This study presents a novel electrochemical biosensor using peptide nucleic acid (PNA) probes for direct and reagentless detection of target sequences. The biosensor demonstrates high selectivity and sensitivity, enabling discrimination of single mismatches.

Area of Science:

Electrochemistry
Biosensor Technology
Molecular Biology

Background:

Peptide nucleic acids (PNAs) offer unique hybridization properties for nucleic acid detection.
Electrochemical biosensors provide sensitive and label-free detection methods.
Developing reagentless biosensors is crucial for simplifying detection protocols.

Purpose of the Study:

To develop and characterize an electrochemical hybridization biosensor utilizing PNA probes.
To investigate the direct and reagentless detection capabilities of the PNA-based biosensor.
To evaluate the biosensor's selectivity and sensitivity for target DNA sequences.

Main Methods:

Covalent attachment of PNA probes onto a quinone-based electroactive polymer.
Electrochemical detection of hybridization events using square wave voltammetry (SWV).

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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

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Last Updated: Jul 4, 2026

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Assessment of sensor response to complementary, non-complementary, and mismatched target sequences.

Main Results:

The PNA-based biosensor exhibited measurable electrochemical changes upon target hybridization.
A significant increase in quinone peak current was observed with complementary targets.
The biosensor demonstrated high selectivity, successfully discriminating single-nucleotide mismatches.

Conclusions:

The developed electrochemical biosensor offers a sensitive and selective platform for nucleic acid detection.
The reagentless and direct detection approach simplifies the hybridization assay.
This PNA-based electrochemical biosensor shows promise for various diagnostic applications.