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Updated: Jul 4, 2026

Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
A lamp light-emitting diode-induced fluorescence detector for capillary electrophoresis
Jing Xu1, Yan Xiong, Shiheng Chen
1Department of Analytical Chemistry and Micro-Instrumentation, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Abstract:
A light-emitting diode-induced fluorescence detector (LED-FD) for capillary electrophoresis was constructed and evaluated. A lamp LED with an enhanced emission spectrum and a band pass filter was used as the excitation light source. Refractive index matching fluid (RIMF) was used in the detection cell to reduce scattering light and the noise level. The limit of detection (LOD) for fluorescein was 1.5 nM (SNR=3). The system exhibited linear responses in the range of 1 x 10(-8) to 5 x 10(-6)M (R=0.999). Application of the lamp LED-FD for the analysis of FITC-labeled ephedra herb extract by capillary electrophoresis was demonstrated.
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