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Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart
Published on: May 19, 2022
Neuropeptide Y (NPY) Y2 receptors of rabbit kidney cortex are largely dimeric
A M Estes1, Y Y Wong, M S Parker
1Department of Pharmacology, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Abstract:
The neuropeptide Y (NPY) Y2 receptors and the pancreatic polypeptide Y4 receptors from rabbit kidney cortex are isolated largely as approximately 180 kDa complexes constituted of one receptor dimer and one G-protein heterotrimer, similar to NPY receptors expressed in the Chinese hamster ovary (CHO) cells. As expected, kidney and CHO cell Y2 dimers are converted into monomers by increasing concentrations of a selective agonist. Prevalence of dimeric Y2 receptors in the kidney could be related to low plasma levels of Y2 agonists, and possibly also to a relatively low concentration of Gi alpha subunits.
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