Studies on the sources of benzo[a]pyrene in grain and aboveground tissues of rice plants

Rice plant pot experiments designed to identify benzo[a]pyrene (B[a]P) sources in plant tissues were conducted in an air-quality controlled greenhouse built to prevent contamination from B[a]P air pollution. Results from quartz sand cultures with control and 50, 100 and 500 microgkg(-1) of B[a]P treatments were compared with those from outdoor field experiments, in which rice plants were exposed to polluted air in the urban area of Shenyang, China. When B[a]P was strictly controlled in both air and quartz sand culture medium, the background values of B[a]P in rice plant tissues were uniformly very low. There was no significant difference of B[a]P contents of rice grain between control and treatments of B[a]P in controlled air quality trials. This indicated that the source of B[a]P in the rice grains is not from any B[a]P in the root culture media. The B[a]P content of rice grain, husk, and stem with leaf sampled from outdoor field was up to 7.33-, 9.21- and 27.10-fold higher than corresponding tissues from air-quality controlled conditions. This indicated that polluted air is the main source of B[a]P in aboveground tissues. Therefore control of B[a]P pollution in ambient air is of prime importance for improving the quality of cereal crops.