Related Experiment Video

Updated: Jul 4, 2026

Imaging of mtHyPer7, a Ratiometric Biosensor for Mitochondrial Peroxide, in Living Yeast Cells
09:47

Imaging of mtHyPer7, a Ratiometric Biosensor for Mitochondrial Peroxide, in Living Yeast Cells

Published on: June 2, 2023

Transmembrane protein 85 from both human (TMEM85) and yeast (YGL231c) inhibit hydrogen peroxide mediated cell death

Giselle Ring1, Chamel M Khoury, Aidan J Solar

  • 1Department of Medicine, McGill University, Montreal, Quebec, Canada.

FEBS Letters
|July 1, 2008
PubMed

Insights

Transmembrane protein 85 (TMEM85) is identified as a novel anti-apoptotic protein. Its expression in yeast enhances cell survival against oxidative stress, revealing a conserved role in apoptosis regulation.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Anti-apoptotic proteins play a crucial role in regulating programmed cell death (apoptosis).
  • The functional roles of many proteins involved in apoptosis remain uncharacterized.

Purpose of the Study:

  • To identify and characterize novel anti-apoptotic proteins.
  • To investigate the function of the uncharacterized transmembrane domain protein 85 (TMEM85) in apoptosis.

Main Methods:

  • Heterologous expression of human TMEM85 in yeast.
  • Growth and viability assays to assess cell death and stress resistance.
  • Analysis of TMEM85 gene splicing and transcript variants.

Main Results:

  • Heterologous expression of human TMEM85 in yeast promoted growth and prevented cell death under oxidative stress.
  • Overexpression of the yeast ortholog (YGL231c) also conferred increased oxidative stress resistance.
  • Human TMEM85 gene exhibits alternative splicing, producing multiple transcripts and proteins.

Conclusions:

  • TMEM85 is a novel conserved anti-apoptotic protein.
  • TMEM85 plays a significant role in cellular protection against oxidative stress.
  • The TMEM85 gene is complex, with alternative splicing contributing to its functional diversity.

More Related Videos

Yeast As a Chassis for Developing Functional Assays to Study Human P5314:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Mitochondrial Respiration Quantification in Yeast Whole Cells07:15

Mitochondrial Respiration Quantification in Yeast Whole Cells

Published on: November 8, 2024

Related Concept Videos

Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
Peroxisomes01:24

Peroxisomes

Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...