Improved pulsed field gel electrophoresis method for Moraxella catarrhalis
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Nevada M Pingault1, Deborah Lehmann, Thomas V Riley
1School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, Perth, Western Australia, Australia. Nevada.Pingault@health.wa.gov.au
Journal of Microbiological Methods
|July 1, 2008
Abstract:
An improved PFGE method for the molecular typing of Moraxella catarrhalis is described. A modified PulseNet method using higher concentrations of EDTA and proteinase K, together with increased reagent volumes and incubation temperatures resulted in improved results and a more rapid turnaround time compared to PFGE methods currently used.

