Related Experiment Video
Updated: Jul 4, 2026

05:16
Establishment of a Murine Pulp Exposure Model with a Novel Mouth-Gag for Pulpitis Research
Published on: October 27, 2023
NaOCl effects on primary and permanent pulp chamber dentin
Ana Flávia Sanches Borges1, Renata Andrade Bittar, Fernanda Miori Pascon
1Latin American Institute of Research and Teaching in Dentistry (ILAPEO), Brazil.
Journal of Dentistry
|July 1, 2008
Summary
Sodium hypochlorite (NaOCl) alters inorganic dentin content in primary and permanent teeth. These chemical changes are detectable by spectroscopy but not always by scanning electron microscopy (SEM) after etching.
Area of Science:
- Dental materials science
- Biomaterials
- Spectroscopy
Background:
- Dentin quality is crucial for dental restorations.
- Understanding differences between primary and permanent dentin is important for clinical applications.
Purpose of the Study:
- To compare the dentin quality of primary and permanent pulp chambers.
- To investigate the effects of sodium hypochlorite (NaOCl) and phosphoric acid treatments on dentin.
Main Methods:
- Fourier-transformed Raman spectroscopy (FT-Raman) and scanning electron microscopy (SEM) were used.
- Dentin fragments from human molars were treated with NaOCl and/or phosphoric acid.
- Cluster analysis and image scoring were performed.
Main Results:
- Primary and permanent dentin showed differences in inorganic content.
- NaOCl treatment significantly altered inorganic content.
- SEM revealed calcospherites in permanent dentin but not primary dentin.
Conclusions:
- NaOCl affects inorganic dentin content irrespective of subsequent phosphoric acid etching.
- SEM did not detect NaOCl-induced chemical changes when followed by etching.

