Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Typical Model Studies01:30

Typical Model Studies

Fluid mechanics model studies often utilize scaled-down systems to predict fluid behavior in full-scale environments, such as river flows, dam spillways, and structures interacting with open surfaces. Maintaining Froude number similarity in river models is crucial, as it replicates surface flow features like wave patterns and velocities.
Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Design Example: Creating a Hydraulic Model of a Dam Spillway01:21

Design Example: Creating a Hydraulic Model of a Dam Spillway

Scaled hydraulic models of dam spillways provide a practical way to replicate and study the intricate flow dynamics of these structures. Often built to a 1:15 ratio, these models allow for observing critical water behavior, such as velocity distribution, flow patterns, and energy dissipation.
Dialysis01:15

Dialysis

Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...
Modeling and Similitude01:12

Modeling and Similitude

Scaled modeling is a fundamental technique in engineering, enabling the study of large and complex systems by creating smaller, manageable replicas that recreate critical characteristics of the original. In hydrology and civil infrastructure, for example, scaled models of dams help analyze water flow, turbulence, and pressure. This method allows for accurate predictions of real-world behavior within a controlled environment, significantly reducing the cost and time involved in full-scale...
Physiological Pharmacokinetic Models: Assumption with Protein Binding01:13

Physiological Pharmacokinetic Models: Assumption with Protein Binding

Physiological models with protein binding in pharmacokinetics offer a sophisticated approach to understanding drug disposition. These models consider drug-protein interactions, enabling them to effectively predict drug concentrations in different organs and tissues. This precision aids in accurate drug dosing, providing a significant advantage over conventional models. A key process within these models is equilibration, which ensures that drug concentrations achieve a steady state within the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-world reported adverse events related to systemic immunomodulating therapy in patients with atopic dermatitis: Results from the TREAT NL (TREatment of ATopic eczema, the Netherlands) registry.

Journal of the European Academy of Dermatology and Venereology : JEADV·2023
Same author

Superior vena cava syndrome presenting as position-dependent periorbital oedema.

BMJ case reports·2018
Same author

Local administration of gentamicin collagen sponge in surgical excision of sacrococcygeal pilonidal sinus disease: a systematic review and meta-analysis of the literature.

Techniques in coloproctology·2015
Same author

Sudden cardiac death after repair of anomalous origin of left coronary artery from right sinus of Valsalva with an interarterial course : Case report and review of the literature.

Netherlands heart journal : monthly journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation·2012
Same author

Human oral mucosa and gingiva: a unique reservoir for mesenchymal stem cells.

Journal of dental research·2012
Same author

Selective recognition of xylene isomers using ZnO-SWNTs hybrid gas sensors.

The Analyst·2012
Same journal

Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture.

Biotechnology and bioengineering·2026
Same journal

LLM-Guided Parameter Optimization for Mechanistic CHO Cell Bioreactor Models.

Biotechnology and bioengineering·2026
Same journal

Three-Dimensional-Printed Polylactic Acid Scaffolds Coated With a Paeonol-Incorporated Gelatin/Bioactive Glass Composite Layer for Enhanced Osteogenic Performance.

Biotechnology and bioengineering·2026
Same journal

Recent Progress in Antimicrobial Peptides (AMPs) Towards Enhanced Selectivity and Reduced Cytotoxicity by Molecular Engineering.

Biotechnology and bioengineering·2026
Same journal

mZVI-Enhanced Mixed Nitrogen Removal in Klebsiella oxytoca via Coordinated Electron Transfer and Metabolic Reprogramming.

Biotechnology and bioengineering·2026
Same journal

Growth Model for Continuous Culture of a Hydrogen-Oxidizing Bacterium, Hydrogenophilus thermoluteolus Strain TH-1.

Biotechnology and bioengineering·2026
See all related articles

Related Experiment Video

Updated: Jul 4, 2026

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing
10:19

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing

Published on: February 13, 2016

Mathematical modeling of affinity ultrafiltration process.

J H Luong1, K B Male, A L Nguyen

  • 1Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada.

Biotechnology and Bioengineering
|August 5, 1988
PubMed
Summary
This summary is machine-generated.

A novel affinity ultrafiltration process effectively purifies trypsin using a specific inhibitor polymer. This method offers high resolution, yield, and scalability for enzyme purification.

More Related Videos

Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification
07:32

Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification

Published on: April 7, 2017

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
12:11

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

Published on: July 9, 2012

Related Experiment Videos

Last Updated: Jul 4, 2026

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing
10:19

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing

Published on: February 13, 2016

Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification
07:32

Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification

Published on: April 7, 2017

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
12:11

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

Published on: July 9, 2012

Area of Science:

  • Biochemical Engineering
  • Protein Purification
  • Separation Science

Background:

  • Enzyme purification is critical for biochemical research and industrial applications.
  • Existing methods for purifying enzymes like trypsin can be complex and costly.
  • There is a need for efficient, scalable, and high-resolution purification techniques.

Purpose of the Study:

  • To develop and validate a novel affinity ultrafiltration process for enzyme purification.
  • To demonstrate the process's effectiveness in purifying trypsin from a mixture.
  • To create a mathematical model for predicting process performance.

Main Methods:

  • Synthesis of a water-soluble polymer functionalized with m-aminobenzamidine, a trypsin inhibitor.
  • Integration of affinity binding with cross-flow ultrafiltration.
  • Development and application of a mathematical model to simulate the purification dynamics.

Main Results:

  • The affinity ultrafiltration process achieved high resolution and high recovery yield.
  • Successful purification of trypsin from a trypsin-chymotrypsin mixture was demonstrated.
  • The developed mathematical model accurately predicted enzyme concentration profiles.

Conclusions:

  • Affinity ultrafiltration is a highly effective and scalable method for purifying trypsin.
  • The synthesized polymer and integrated filtration system provide a robust purification solution.
  • The mathematical model aids in optimizing and understanding the process dynamics.