Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bioreactor Controls-III

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

Role of Microtubules in Cell Wall Deposition

Role of Microtubules in Cell Wall Deposition

Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of disassembly and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Pion, kaon, and proton production in central Pb-Pb collisions at sqrt[s(NN)] = 2.76 TeV.

Physical review letters·2013

Same author

Measurement of the cross section for electromagnetic dissociation with neutron emission in Pb-Pb collisions at sqrt[s(NN)] = 2.76 TeV.

Physical review letters·2013

Same author

Directed flow of identified particles in Au+Au collisions at √[SNN]=200 GeV at RHIC.

Physical review letters·2012

Same author

Production of muons from heavy flavor decays at forward rapidity in pp and Pb-Pb collisions at sqrt[s(NN)]=2.76 TeV.

Physical review letters·2012

Same author

J/ψ suppression at forward rapidity in Pb-Pb collisions at √s(NN) = 2.76 TeV.

Physical review letters·2012

Same author

Effective population size in eusocial Hymenoptera with worker-produced males.

Heredity·2012

Search research articles

Related Experiment Video

Updated: Jul 4, 2026

Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

Published on: June 13, 2014

Variation in cellulase-constituting components from Trichoderma reesei with agitation intensity.

S Mukataka¹, N Kobayashi, S Sato

¹Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305, Japan.

Biotechnology and Bioengineering

|September 5, 1988

Summary

Agitation intensity significantly impacts Trichoderma reesei QM9414 cellulase production. Optimal agitation speeds vary for filter paper activity (FPA), carboxymethylcellulase (CMCase), and beta-glucosidase, with high agitation reducing enzyme yields.

More Related Videos

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

Published on: August 13, 2011

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

Related Experiment Videos

Last Updated: Jul 4, 2026

Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

Published on: June 13, 2014

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

Published on: August 13, 2011

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

Area of Science:

Biotechnology
Enzyme Engineering
Microbial Physiology

Background:

Trichoderma reesei QM9414 is a key microorganism for industrial enzyme production.
Cellulase production is influenced by various fermentation parameters.
Understanding agitation effects is crucial for optimizing enzyme yields.

Purpose of the Study:

To investigate the impact of agitation intensity on cellulase production by Trichoderma reesei QM9414.
To determine optimal agitation conditions for different cellulase components.
To correlate enzyme production with morphological changes under varying agitation.

Main Methods:

Cultivation of Trichoderma reesei QM9414 under controlled dissolved oxygen (>3 ppm).
Systematic variation of agitation intensity (tip velocity from 0.3 to 1.7 m/s).
Measurement of filter paper activity (FPA), CMCase, and beta-glucosidase activity.
Microscopic observation of mycelial morphology (pellet formation, hyphal fragmentation).

Main Results:

Cellulase production showed a strong dependence on agitation intensity, with varying optima for FPA (1.0 m/s), CMCase (0.7 m/s), and beta-glucosidase (1.4 m/s).
High agitation (>1.3 m/s) significantly reduced all cellulase components.
Mild agitation (0.3-1.0 m/s) promoted pellet formation, while intense agitation led to hyphal fragmentation.

Conclusions:

Agitation intensity is a critical factor in optimizing cellulase production by T. reesei.
Specific agitation regimes are required for maximizing individual cellulase components.
Morphological changes, such as pelletization and hyphal fragmentation, are directly linked to enzyme production efficiency.