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Related Concept Videos

Fed-Batch Culture01:23

Fed-Batch Culture

Fed-batch culture is a widely used bioprocessing strategy combining aspects of batch culture with controlled substrate feeding to optimize cell growth and product formation. In this semi-closed system, nutrients are strategically added during fermentation, while the accumulated products and biomass remain within the bioreactor until the end of the operation. This controlled addition of substrates allows for better management of growth kinetics, nutrient limitation, and metabolite...
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Fermentation is a foundational biotechnological process used to produce pharmaceuticals, biofuels, enzymes, and food additives. Among industrial strategies, batch and continuous fermentation are the two most widely applied. Although both rely on microbial conversion of substrates into desired products, they differ markedly in operation, productivity, and suitability for specific applications.Batch fermentation occurs in a closed system in which nutrient media and inoculum are added at the...
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The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
Bioreactor Controls-II01:18

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In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Bioreactor Controls-III01:22

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Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
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Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...

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Operation of a Benchtop Bioreactor
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An algorithm for operating a fed-batch fermentor at optimum specific-growth rate.

P Agrawal1, G Koshy, M Ramseier

  • 1Department of Chemical Engineering, University of California, Santa Barbara, California 93106, USA.

Biotechnology and Bioengineering
|January 5, 1989
PubMed
Summary
This summary is machine-generated.

This study introduces a novel algorithm for fed-batch fermentation, optimizing the specific fermentation rate without needing nutrient measurements. The model-independent approach ensures effective cell-mass production even with imprecise data.

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Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Fermentation Technology

Background:

  • Fed-batch fermentation is a key bioprocess for maximizing product yield.
  • Real-time monitoring of nutrient concentrations is often complex and costly.
  • Optimizing specific fermentation rates is crucial for efficient bioprocessing.

Purpose of the Study:

  • To propose a novel algorithm for operating fed-batch fermentors.
  • To achieve optimal specific fermentation rates without on-line nutrient measurement.
  • To demonstrate the algorithm's robustness and efficacy in cell-mass production.

Main Methods:

  • Development of a model-independent algorithm.
  • Utilizing on-line estimates of the specific fermentation rate for control.
  • Simulation studies for optimizing cell-mass production in fed-batch systems.

Main Results:

  • The proposed algorithm effectively operates fed-batch fermentors at optimal specific fermentation rates.
  • The method does not require on-line measurement of nutrient concentration.
  • The algorithm performs well even with inaccurate estimates of product yields and fermentation rates.

Conclusions:

  • The developed algorithm offers a simplified and robust approach to fed-batch fermentation control.
  • This method enhances process efficiency and reduces operational complexity.
  • It is effective across diverse fermentation scenarios for optimizing cell-mass production.