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Related Concept Videos

Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Scale-Up Processes01:14

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The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
Bioreactor Controls-I01:28

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...

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Modelling Xanthomonas campestris batch fermentations in a bubble column.

A Pons1, C G Dussap, J B Gros

  • 1Laboratoire de Génie Chimique Biologique, Université Blaise Pascal, 63170 Aubiere, France.

Biotechnology and Bioengineering
|January 20, 1989
PubMed
Summary

This study models Xanthomonas campestris fermentation, finding oxygen levels critically impact xanthan production rates and yields. Improved models account for dissolved oxygen

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Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Microbial Fermentation

Background:

  • Xanthomonas campestris fermentation is crucial for xanthan gum production.
  • Existing models like Luedeking-Piret inadequately describe xanthan yield and substrate consumption over time.
  • Oxygen availability is a known factor influencing microbial metabolism.

Purpose of the Study:

  • To develop a more accurate model for Xanthomonas campestris batch fermentation.
  • To establish rate and yield expressions for biomass, xanthan, and substrate consumption.
  • To investigate the impact of dissolved oxygen tension on fermentation kinetics.

Main Methods:

  • Batch fermentation of Xanthomonas campestris in a bubble column.
  • Application of the logistic rate equation for microbial growth.
  • Development of new rate and yield expressions considering metabolic pathways and energetic balance.
  • Modeling oxygen limitation on respiration and oxidative phosphorylation (P/O ratio).

Main Results:

  • Microbial growth followed the logistic rate equation with mu(M) = 0.5 h(-1).
  • Experimental yields and specific rates decreased over time, indicating Luedeking-Piret model inadequacy.
  • Dissolved-oxygen tension significantly affected xanthan synthesis and glucose assimilation.
  • Developed model shows specific rates and yields are dependent on dissolved-oxygen tension.

Conclusions:

  • Oxygen limitation is a key factor in Xanthomonas campestris fermentation, affecting both growth and product formation.
  • The proposed model, incorporating oxygen's role, provides a more accurate representation of fermentation kinetics.
  • Optimizing oxygen transfer is critical for maximizing xanthan production efficiency in fermentors.