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Plasmids

Plasmids

Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...

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Related Experiment Video

Updated: Jul 4, 2026

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

Published on: December 27, 2024

Data analysis of plasmid maintenance in a CSTR.

K Y San¹, A E Weber

¹Department of Chemical Engineering, Rice University, Houston, Texas 77251-1892, USA.

Biotechnology and Bioengineering

|January 20, 1989

Summary

A new method quantifies plasmid loss and growth rate differences in recombinant cells. This simple procedure, based on a known model, accurately analyzes chemostat data for plasmid maintenance studies.

Area of Science:

Biotechnology
Microbial Genetics
Chemical Engineering

Background:

Plasmid maintenance in recombinant cells is crucial for stable production.
Analyzing plasmid loss and growth dynamics in chemostats requires robust methods.
Existing models may not fully capture experimental data nuances.

Purpose of the Study:

To develop a simple procedure for processing experimental data from plasmid maintenance studies.
To provide quantitative insights into plasmid loss rates and growth rate differences.
To validate the method using simulations and existing experimental data.

Main Methods:

Utilized a procedure based on the Imanaka and Aiba model.
Applied the method to analyze recombinant cells in a chemostat under nonselective conditions.

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Last Updated: Jul 4, 2026

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

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Published on: December 27, 2024

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

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Use of In Vivo Assembly for High-efficiency Plasmid Construction

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Evaluated the method's performance through simulation studies and real-world data analysis.

Main Results:

The developed procedure quantifies plasmid loss rate and growth rate differences.
Simulation studies confirmed the method's effectiveness.
The two-parameter model demonstrated an excellent fit to previously reported experimental data.

Conclusions:

The proposed procedure offers a straightforward approach for analyzing plasmid stability data.
The method provides valuable quantitative information for optimizing recombinant cell cultures.
The model accurately represents plasmid dynamics in chemostat systems.