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Integration of bioconversion and downstream processing: starch hydrolysis in an aqueous two-phase system
M Larsson1, V Arasaratnam, B Mattiasson
1Department of Biotechnology, Chemical Center, University of Lund, PO Box 124, S-221 00 Lund, Sweden.
Biotechnology and Bioengineering
|February 5, 1989
Summary
This study integrates bioconversion with downstream processing using an aqueous two-phase system and ultrafiltration. This method enables continuous glucose production from starch, recycling enzymes and polymers while preventing membrane clogging.
Area of Science:
- Biochemical Engineering
- Bioprocess Technology
- Separation Science
Background:
- Bioprocess productivity can be enhanced by integrating bioconversion with downstream processing.
- Continuous operation of bioconversions is desirable for increased efficiency.
Purpose of the Study:
- To demonstrate the integration of an aqueous two-phase system with ultrafiltration for continuous bioprocessing.
- To achieve continuous glucose production from starch using starch-degrading enzymes.
Main Methods:
- Utilized an aqueous two-phase system combined with ultrafiltration for starch to glucose conversion.
- Employed alpha-amylase and glucoamylase in a mixer-settler reactor for continuous operation over 8 days.
- Recycled phase-forming polymers and enzymes, monitored enzyme activities, and optimized process parameters (pH, temperature, ionic strength).
Main Results:
- A continuous stream of glucose in buffer solution was successfully obtained.
- Phase-forming polymers and enzymes were effectively recycled, preventing ultrafiltration membrane clogging.
- Enzymes exhibited higher activity and stability in the polymer-containing medium compared to buffer solutions.
Conclusions:
- The integrated aqueous two-phase system and ultrafiltration approach is effective for continuous bioconversion.
- This method enhances bioprocess productivity and allows for enzyme and polymer recycling.
- The system demonstrates robustness and potential for industrial application in continuous glucose production.
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