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Updated: Jul 4, 2026
09:14
Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine
Published on: February 16, 2018
Invertase-catalyzed reactions in alcoholic solutions
B Selisko1, R Ulbrich, A Schellenberger
1Department of Biotechnology, Martin-Luther University, DDR-4020 Halle, German Democratic Republic.
Biotechnology and Bioengineering
|April 25, 1990
Summary
Invertase enzyme catalyzes the formation of alkyl beta-D-fructosides from sucrose. Alcohol chain length impacts yield, with shorter chains like methanol yielding more products.
Area of Science:
- Biochemistry
- Enzymology
- Carbohydrate Chemistry
Background:
- Enzymatic synthesis offers a green route for producing valuable carbohydrate derivatives.
- Invertase, a key enzyme in sucrose metabolism, has potential applications in biocatalysis.
Purpose of the Study:
- To investigate the enzymatic synthesis of alkyl beta-D-fructosides using invertase.
- To determine the influence of alcohol type, concentration, and reaction time on product yield and selectivity.
Main Methods:
- Enzymatic reactions using invertase with sucrose in aqueous alcohol solutions (methanol, ethanol, n-propanol).
- Kinetic analysis of sucrose conversion, alcoholysis, and hydrolysis reactions.
- Analysis of product distribution using chromatographic techniques.
Main Results:
- Alkyl beta-D-fructosides are formed via competitive alcoholysis and hydrolysis reactions.
- Product yield and sucrose conversion rate decrease with increasing alcohol chain length (methanol > ethanol > n-propanol).
- Invertase can also synthesize alkyl beta-D-fructosides from fructose in alcoholic solutions.
Conclusions:
- The formation of alkyl beta-D-fructosides is controllable by reaction conditions and alcohol choice.
- Invertase-mediated synthesis provides a pathway for producing functionalized fructosides.
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