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Published on: February 27, 2020
Versatile platform employing desorption electrospray ionization mass spectrometry for high-throughput analysis
Xiaoxiao Ma1, Mengxia Zhao, Ziqing Lin
1Department of Chemistry, Key Laboratory for Atomic and Molecular Nanosciences of Education Ministry, Tsinghua University, 100084, Beijing, PR China.
Analytical Chemistry
|July 1, 2008
Summary
A novel high-throughput analysis system utilizing 16 parallel capillaries and desorption electrospray ionization (DESI) achieves 600 samples/hour. This system enables rapid analysis and online derivatization for mass spectrometry.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Instrumentation
Background:
- High-throughput analysis is crucial for accelerating scientific discovery.
- Desorption electrospray ionization (DESI) offers versatile ambient ionization capabilities.
- Integrating multiple sample introduction points enhances analytical throughput.
Purpose of the Study:
- To develop and evaluate a simple, easy-to-build high-throughput analysis system.
- To expand the functional capabilities of DESI for rapid sample analysis.
- To demonstrate the system's performance for quantitative analysis and online derivatization.
Main Methods:
- Construction of a system comprising a 16-channel capillary device, a DESI source, and a linear ion trap mass spectrometer.
- Automated horizontal translation of the multichannel device for sequential sample analysis.
- Utilizing nebulizing gas to drive liquid samples, ionize them via DESI, and transfer to the mass spectrometer.
Main Results:
- Achieved an analytical throughput of 600 samples per hour, analyzing 16 capillaries within 1.6 minutes.
- Successfully demonstrated reactive DESI for online derivatization feasibility.
- Obtained relative standard deviations of 7.6% (single capillary) and 12.3% (five capillaries).
- Confirmed linear response for 1,3-diethyl-1,3-diphenylurea (DDU) with a correlation coefficient of 0.9971.
Conclusions:
- The developed system provides a highly efficient and simple solution for high-throughput mass spectrometry analysis.
- The system effectively integrates DESI with multichannel sample introduction for enhanced performance.
- This platform is suitable for rapid quantitative analysis and facilitates online derivatization experiments.
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