Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 4, 2026

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

Published on: October 6, 2018

Bioartificial liver systems: why, what, whither?

Jörg C Gerlach¹, Katrin Zeilinger, John F Patzer Ii

¹Department of Surgery & Bioengineering, McGowan Institute for Regenerative Medicine, Bridgeside Point Bldg., 100 Technology Drive, Suite 225, Pittsburgh, PA 15219-3130, USA. jgerlach@piitt.edu

Regenerative Medicine

|July 1, 2008

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Kinetic Analysis of Lidocaine Elimination by Pig Liver Cells Cultured in 3D Multi-Compartment Hollow Fiber Membrane Network Perfusion Bioreactors.

Bioengineering (Basel, Switzerland)·2021

Same author

Conditioned Medium from Human Amnion-Derived Mesenchymal Stromal/Stem Cells Attenuating the Effects of Cold Ischemia-Reperfusion Injury in an In Vitro Model Using Human Alveolar Epithelial Cells.

International journal of molecular sciences·2021

Same author

Effects of Mesenchymal Stem Cell Coculture on Human Lung Small Airway Epithelial Cells.

BioMed research international·2020

Same author

Comparative study of the production of soluble factors in human placenta-derived mesenchymal stromal/stem cells grown in adherent conditions or as aggregates in a catheter-like device.

Biochemical and biophysical research communications·2019

Same author

Isolation and Characterization of a Human Fetal Mesenchymal Stem Cell Population: Exploring the Potential for Cell Banking in Wound Healing Therapies.

Cell transplantation·2019

Same author

Effect of inoculum density on human-induced pluripotent stem cell expansion in 3D bioreactors.

Cell proliferation·2019

Same journal

Industry updates in advanced therapy medicinal products and regenerative medicine - May 2026.

Regenerative medicine·2026

Same journal

Ethical, legal, and social issues associated with human fetal tissue research in Japan.

Regenerative medicine·2026

Same journal

The future of hematopoietic stem cell and stem cell gene therapy for metabolic diseases.

Regenerative medicine·2026

Same journal

Exploring gene therapy for developmental and epileptic encephalopathies (DEEs): possibilities or promises?

Regenerative medicine·2026

Same journal

Industry updates in advanced therapy medicinal products and regenerative medicine - April 2026.

Regenerative medicine·2026

Same journal

Optimizing strategies in tendon tissue engineering through effective scaffold design: overview of recent advancements.

Regenerative medicine·2026

See all related articles

Developing a novel bioreactor for scalable liver cell expansion is crucial for regenerative medicine. This technology aims to provide a functional cell source for treating acute liver failure, improving patient outcomes.

Area of Science:

Regenerative Medicine
Hepatology
Biotechnology

Background:

Acute liver failure is a life-threatening condition with limited treatment options beyond liver transplantation.
Current detoxification therapies (MARS, Prometheus) and bioartificial liver systems have not demonstrated significant clinical efficacy in Phase III trials.
Cell transplantation faces challenges due to cell scarcity and limited practicability for widespread use.

Purpose of the Study:

To address the need for a scalable, functional cell source for liver disease treatment.
To introduce a novel bioreactor design that mimics the native liver microenvironment.
To facilitate the expansion of various liver-related cell types for therapeutic applications.

Main Methods:

A novel bioreactor design was developed to closely replicate the native liver cell environment.

More Related Videos

Fabrication of Decellularized Spleen Matrix Derived from Rats

Fabrication of Decellularized Spleen Matrix Derived from Rats

Published on: February 9, 2024

Decellularization and Recellularization of Whole Livers

Decellularization and Recellularization of Whole Livers

Published on: February 4, 2011

Related Experiment Videos

Last Updated: Jul 4, 2026

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

Published on: October 6, 2018

Fabrication of Decellularized Spleen Matrix Derived from Rats

Fabrication of Decellularized Spleen Matrix Derived from Rats

Published on: February 9, 2024

Decellularization and Recellularization of Whole Livers

Decellularization and Recellularization of Whole Livers

Published on: February 4, 2011

The bioreactor system is designed for scalability, from microscopic to clinical volumes.

The bioreactor maintains a consistent local cell environment across different scales.

Main Results:

The described bioreactor design enables the study of primary liver cells, cell lines, and stem/progenitor cells.
The system is easily scalable, supporting cell expansion from <1 ml to approximately 600 ml.
The bioreactor maintains the native liver cell environment throughout its operational range.

Conclusions:

The novel bioreactor offers a promising platform for generating expandable liver cell sources.
This technology is vital for advancing regenerative medicine approaches to liver disease.
The scalable design supports diverse applications, from research to clinical cell therapy.