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Differences between OK and LLC-PK1 cells: cystine handling.
1Division of Biochemical Development and Molecular Diseases, Children's Hospital of Philadelphia, Pennsylvania.
The American Journal of Physiology
|July 1, 1991
Summary
Opossum kidney (OK) and porcine kidney (LLC-PK1) cells exhibit distinct cystine transport mechanisms. OK cells utilize apical transport influenced by lysine, while LLC-PK1 cells use basolateral transport affected by glutamate.
Area of Science:
- Cell Biology
- Biochemistry
- Renal Physiology
Background:
- Kidney cells play a crucial role in amino acid transport.
- Understanding cystine transport is vital for renal health and disease.
- Distinct cell lines offer models to study specific transport mechanisms.
Purpose of the Study:
- To compare biochemical characteristics and cystine transport systems in cultured opossum kidney (OK) and porcine kidney (LLC-PK1) cells.
- To identify differences in protein content, DNA, and cystine uptake regulation.
- To determine the membrane localization and inhibitory factors of cystine transport in each cell line.
Main Methods:
- Culturing of OK and LLC-PK1 cells.
- Measurement of protein content and DNA per cell.
- Assessment of cystine uptake rates and inhibition studies.
- Determination of transport localization (apical vs. basolateral).
Main Results:
- OK cells have lower protein content but increased cystine uptake at confluence, inhibited by lysine.
- LLC-PK1 cells show decreased cystine uptake at confluence, inhibited by glutamate.
- Cystine transport is apical in OK cells and basolateral in LLC-PK1 cells.
- OK cells have a smaller cysteine pool compared to LLC-PK1 cells.
Conclusions:
- OK cells model the cystine-dibasic amino acid transport system.
- LLC-PK1 cells model the cystine-glutamate antiporter system.
- These distinct cell models facilitate research into cystine transport system development and structure.