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Updated: Jul 4, 2026

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
Published on: July 22, 2011
[Study on the molecular typing of Enterobacter sakazakii with pulsed-field gel electrophoreses]
Xiaoyan Pei1, Yunchang Guo, Xiumei Liu
1Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention,Beijing 100050, China.
Objective:
To analyze the fingerprint of Enterobacter sakazakii, twenty-nine isolates and two type strains were analyzed with the pulsed-field gel electrophoreses (PFGE).
Methods:
All of strains were digested with restriction endonucleases Xba I and Spe I respectively, and the PFGE patterns were analyzed with BioNumerics software.
Results:
PFGE with restriction endonucleases Xba I and Spe I gave 30 kinds of PFGE patterns respectively. Twenty-nine strains of Enterobacter sakazakii gave different patterns except ES 004 and ES 005. ES 004 and ES 005, which were isolated from the infant formula with same brand and same lot but different package, showed the same PFGE pattern with endonucleases Xba I and Spe I. According to the analysis of BioNumerics software, there is no significant correlation between PFGE patterns and source information of corresponding samples except ES 004 and ES 005.
Conclusion:
Though the average number of PFGE bands with Xba I was less than that with Spe I, it proved that PFGE with Xba I still has a high degree of discrimination. The above two PFGE methods both can be used to the molecular typing and tracing of Enterobacter sakazakii.
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