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Updated: Jul 4, 2026

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Published on: February 1, 2014
[Study on the rapid detection method of Enterotoxigenic E. coli by polymerase chain reaction technology]
Yufeng Li1, Juan Dai, Honglu Liu
1College of Bioengineering, Xihua University, Chengdu 610039, China.
Objective:
To establish the rapid detection method of Enterotoxigenic E. coli (ETEC) by polymerase chain reaction (PCR) technology for characteristic primers.
Methods:
The primer of LT gene from Enterotoxigenic E. coli was designed and the condition of PCR was optimized to identify ETEC with PCR amplification for 16 samples.
Results:
all samples could not be amplified except ETEC with special primer.
Conclusion:
The technology of ETEC determinated by PCR could be easy, rapid and accurate.
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