Related Experiment Video

Updated: Jul 4, 2026

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
09:00

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies

Published on: May 22, 2012

[Some practical notes about performing polymerase chain reaction and electrophoretic fractionation of amplificated

I A Shilov, E Iu Zemskova, N L Kaganova

    Sudebno-Meditsinskaia Ekspertiza
    |July 1, 2008
    PubMed

    More Related Videos

    Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems07:35

    Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

    Published on: June 14, 2021

    Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization12:37

    Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

    Published on: April 14, 2016

    Related Concept Videos

    PCR01:32

    PCR

    Overview
    PCR - Polymerase Chain Reaction01:32

    PCR - Polymerase Chain Reaction

    Overview
    Real Time RT-PCR02:57

    Real Time RT-PCR

    Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
    The real-time quantification of the number of amplified products is...
    DNA Agarose Gel Electrophoresis02:35

    DNA Agarose Gel Electrophoresis

    Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
    Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
    In cloning experiments, both the insert and vector DNA...