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Published on: June 8, 2012
[Effect of Auricularia auricula polysaccharide on PBMCs proliferation and cytokine expression in vitro]
Jiang-cheng Zuo1, Yi-qin Zeng, Xiao-wen He
1The First College of Clinical Medical Science, Three Gorges University, Yichang 443002, China. jichzuo@gmail.com
Objective:
To investigate the proliferation and cytokine expression of Auricularia auricula polysaccharide (AAP) on peripheral blood monnuclear cells (PBMCs) in vitro.
Methods:
AAP was obtained by water extraction and alcohol precipitation, and purified by DEAE-cellulose chromatogram. PBMCs were treated with AAP at different levels. The Cell Counting Kit-8 (CCK-8) was used to determine the proliferation of PBMCs. The concentration of TNF-alpha and TFN-gamma in supernatant were investigated by ELISA.
Results:
The three fractions of AAP could stimulate PBMCs proliferation at the range of 100--800 microg/ml, and AAP2 enhanced significantly the level of TNF-alpha and IFN-gamma in supernatant of PBMCs (P < 0.05, P < 0.01).
Conclusion:
AAP is an immunomodulator and can enhance the immunomodulatory activity through TNF-alpha and TFN-gamma.
