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[Study of flavonoids distribution in various populations of Prunella vulgaris]
Li Liao1, Qiao-Sheng Guo, Li Liu
1Institute of Chinese Medicinal Materials, Nanjin Agricultural University, Nanjing 210095, China.
Objective:
To study the extraction flavonoids distribution in different parts of Prunella vulgaris from different populations.
Method:
The optimal extraction condition was selected by response surface method (RSM). The amounts of flavonoids were determined by colorimetric method.
Result:
The optimum extraction condition was using 35% ethanol at 25 times of sawple volume, refluxing at 87 degrees C for 3.5 h. The amount of flavonoids was 2.16% -10.29% in P. vulgaris. The content of flavonoids was the highest in leaf while that in root was the lowest. And the content of flavonoids in spike after removed seeds was 27.6% higher than the one reserved seeds.
Conclusion:
The RSM was feasible for optimum extraction condition and the amount of flavonoids showed a signiticant regional distribution pattern in various populations of P. vulgaris.
