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Updated: Jul 4, 2026
Isolation and Culture of Endothelial Cells from the Embryonic Forebrain
Published on: January 23, 2014
[Isolation, culture and characterization of endothelial cells in infantile hemangioma]
Gang Ma1, Xiao-Xi Lin, Yun-Bo Jin
1Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200011, China.
Objective:
To establish a method for the reliable isolation and culture of infantile hemangioma endothelial cells (HemECs) in vitro.
Methods:
Proliferative hemangioma specimens were digested by collagenase to form a single cell suspension. The HemECs were isolated using anti-CD31 coated dynabeads. The CD31+ cells were grown in fibronectin coated dishes. HemECs were identified by morphological characteristics and immunocytochemistry. The cells were also examined for their ability to intake LDL.
Results:
The method enabled the rapid isolation of HemECs that demonstrated typical endothelial cobblestone morphology in culture. The cells were positively stained for CD31, vWF. They also were labeled with DiI-Ac-LDL.
Conclusions:
This technique can effectively isolate endothelial cells from the proliferative hemangiomas. These cells could be further used to research the mechanism of proliferation and degeneration of infantile hemangioma.
