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Updated: Jul 4, 2026

A Method for Obtaining Serial Ultrathin Sections of Microorganisms in Transmission Electron Microscopy
Published on: January 17, 2018
Processing scarce biological samples for light and transmission electron microscopy
1National Neuroscience Institute, Singapore. obgpjt@nus.edu.sg
Abstract:
Light microscopy (LM) and transmission electron microscopy (TEM) aim at understanding the relationship structure-function. With advances in biology, isolation and purification of scarce populations of cells or subcellular structures may not lead to enough biological material, for processing for LM and TEM. A protocol for preparation of scarce biological samples is presented. It is based on pre-embedding the biological samples, suspensions or pellets, in bovine serum albumin (BSA) and bis-acrylamide (BA), cross-linked and polymerized. This preparation provides a simple and reproducible technique to process biological materials, present in limited quantities that can not be amplified, for light and transmission electron microscopy.
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