Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Chromatin Packaging02:21

Chromatin Packaging

Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
Chromatin Packaging01:32

Chromatin Packaging

Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
Chromosome Structure02:40

Chromosome Structure

A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An encyclopedia of human enhancer-gene regulatory interactions.

Nature·2026
Same author

Decentralized ADMM for factorization-based Low-rank matrix estimation.

Neural networks : the official journal of the International Neural Network Society·2026
Same author

Prioritizing peptides for targeted mass spectrometry experiments using deep learning.

bioRxiv : the preprint server for biology·2026
Same author

Embryo-scale Visual Cell Sorting reveals a conserved transcriptomic signature of nucleolar size linked to proteostasis.

bioRxiv : the preprint server for biology·2026
Same author

Prediction and functional interpretation of inter-chromosomal genome architecture from DNA sequence with TwinC.

Nature communications·2026
Same author

Natural variations in GhNF-YB3 contribute to seed cotton yield by modulating source-to-sink sucrose allocation.

The Plant cell·2026

Related Experiment Video

Updated: Jul 4, 2026

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

Automated mapping of large-scale chromatin structure in ENCODE.

Heng Lian1, William A Thompson, Robert Thurman

  • 1Division of Mathematical Sciences, SPMS, Nanyang Technological University, Singapore.

Bioinformatics (Oxford, England)
|July 2, 2008
PubMed
Summary

A new Bayesian model reveals four distinct chromatin accessibility states from DNaseI sensitivity data. Hypersensitive sites, enriched in functional elements, are concentrated within larger intermediate sensitivity domains.

More Related Videos

Mapping Mammalian 3D Genome Interactions with Micro-C-XL
11:41

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

Published on: November 3, 2023

A Multilabel Single Molecule Localization Microscopy Protocol for Investigation of Chromatin in the Dense Nuclear Environment
08:49

A Multilabel Single Molecule Localization Microscopy Protocol for Investigation of Chromatin in the Dense Nuclear Environment

Published on: June 5, 2026

Related Experiment Videos

Last Updated: Jul 4, 2026

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

Mapping Mammalian 3D Genome Interactions with Micro-C-XL
11:41

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

Published on: November 3, 2023

A Multilabel Single Molecule Localization Microscopy Protocol for Investigation of Chromatin in the Dense Nuclear Environment
08:49

A Multilabel Single Molecule Localization Microscopy Protocol for Investigation of Chromatin in the Dense Nuclear Environment

Published on: June 5, 2026

Area of Science:

  • Genomics
  • Computational Biology
  • Epigenetics

Background:

  • Genome-wide chromatin structure analysis is crucial for understanding gene regulation.
  • The DNaseI assay provides a comprehensive view of chromatin accessibility.
  • Previous methods like Hidden Markov Models (HMMs) have limitations in characterizing complex chromatin data.

Purpose of the Study:

  • To apply a Bayesian hierarchical change-point model (CPM) to DNaseI sensitivity data from the ENCODE project.
  • To characterize genome-wide chromatin accessibility features more completely.
  • To identify distinct classes of chromatin regions based on DNaseI sensitivity.

Main Methods:

  • Utilized a Bayesian hierarchical change-point model (CPM), a generalization of HMMs.
  • Analyzed tiled microarray DNaseI sensitivity data from the ENCODE project.
  • Developed a four-state model to describe local chromatin segments.

Main Results:

  • A four-state CPM provided a better fit to the data than HMMs.
  • Identified four classes: major/minor DNaseI hypersensitive sites (DHSs), intermediate sensitivity, and insensitive regions.
  • Regions of intermediate and hypersensitive sites are significantly enriched in functional elements like genes and histone modifications.

Conclusions:

  • Chromatin accessibility can be effectively modeled using a four-state CPM.
  • DNaseI hypersensitive sites are localized within larger domains of intermediate accessibility.
  • The model provides a refined understanding of chromatin structure and its relation to genomic function.